Isolation, selection, and applying of probiotics bacteria for the production of safety chicken meat
Nguyen Huu Hiep1, Nguyen Phuoc Hien2, Tran thi Ai Van2 and Mai thi Ly2
- Biotechnology Research and Development Institute, Cantho University, Vietnam
- Biotechnology program of Biotechnology Research and Development Institute, Cantho University, Vietnam
ABSTRACT
Poultry production is an important economic area in Vietnam. Antibiotics have been used effectively to control enteric pathogens in livestock production. However, antibiotics residues in chicken meat is a severe concern. Probiotic is a good alternative in poultry production. Thirty six strains were isolated from human breast milk and goat milk. Most of them are cocci shape. All of them are gram positive and non motile. Seventeen strains could survive at pH 3 after 3 hours. All isolated bacteria could resist to Streptomycin and Cephalexin at the concentration of 256mg/L, two strains could resist to Penicillin V at the concentration of 256mg/L, seven strains resist to Tetracycline at the concentration of 256mg/L and seven strains resist to Ampicillin at the concentration of 128mg/L. Two promising strains were identified as Enterococcus faecalis and Enterococcus faecium. These two strains were used as probiotic supplement in poultry production. The results showed that chicken applied antibiotics had antibiotic residues higher than those of permission standard while chicken applied probiotics had no evidence of antibiotic residues. The results from this study confirm that probiotics can be used as a good alternative technique in poultry production.